Iso-acoustic focusing for label-free cell profiling and isolation
The following proposed master’s thesis subjects are all related to a research program where we will measure the acoustic properties of cells using a newly developed method called iso-acoustic focusing. This method enables label-free identification and subsequent isolation of cells from e.g. blood samples. This can for example be useful for continuous monitoring of disease progression in patients and can lead to dialysis-like treatments to selectively deplete target cells. You will work independently but with frequent interactions with the supervisor in the form of individual meetings as well as project meetings for the overall research project.
An overview of our research relating to acoustic cell separation can be found on our research pages.
Measuring the acoustic properties of cancer cells
In this project the acoustic properties of cancer cells will be studied and compared to the properties of other cells found in blood.
Fast multicolor fluorescence imaging and analysis of cells in acoustophoresis microchannels
In this project a sensitive camera and a LED light source will be configured to image cells in acoustophoresis flow channels. Cell position, multicolor fluorescence intensities and morphology will be analyzed to map the acoustic and optical properties of individual cells.
3D astigmatism imaging for visualization of 3D flow in microfluidic channels
In this project a microscope will be equipped with a cylindrical lens to image fluorescent tracer particles inside an acoustophoresis microchannel. The cylindrical lens introduces a shape distortion that depends on the height coordinate of the imaged particles. By tracking the particles in a sequence of images the 3D fluid flow inside the channel can be mapped to better understand how an acoustic field interacts with an acoustic impedance gradient.
Absorbance and fluorescence measurements in low volume flow cells
In this project a method will be developed to measure the constituents (molecules and cells) of a liquid using a low volume flow cell using compact optical or electrical readout. This will then be an integral part of the sample inlet feed to the iso-acoustic focusing chip and has further potential for use in process monitoring in microfluidic systems.